鄰近閃爍分析
- 分類：體外生物學分析方法
- 發布時間：2020-08-14 00:00:00
- 訪問量：0
概要:The use of appropriate radioisotopes and scintillant incorporated microbeads allows the scintillation proximity assay (SPA) to become truly homogeneous and high-throughput. As there are ways of capturing radiolabelled molecules either directly or via a binding partner, the assay could be used for sensitive detection of enzymatic process as well as protein-protein interaction. For the former, we have developed a SPA assay for a methyltransferase. The ability of the enzyme in putting a radiolabelled methyl group onto a biotinylated peptide is tested in the presence of an inhibitor. SPA is relatively a versatile technique and has broad assay application, Biortus looks forward to utilise the full potential of this assay technology for our clients.
詳情
鄰近閃爍分析（SPA）選用合適的放射性同位素和結合了閃爍劑的微珠，使其實現了真正的均一性和高通量。 由于有許多直接或者間接（固定配體）捕獲放射性標記分子的方式，該方法可以靈敏的檢測酶促反應過程以及蛋白質-蛋白質的相互作用。對于前者，我們已經開發了甲基轉移酶的SPA分析方法， 在抑制劑存在的條件下測試酶將放射性標記的甲基轉移到生物素化多肽上的能力。 SPA是一種相對多樣化的技術，并且具有廣泛的分析應用，佰翱得期待利用這種分析技術為我們的客戶提供滿意的服務。
佰翱得鄰近閃爍分析(SPA) 實例：
