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激酶檢測
- 分類:體外生物學分析方法
- 發布時間:2020-08-14 00:00:00
- 訪問量:0
概要:Capillary electrophoretic separation prior to fluorescence detection in a Caliper LabChip mobility shift assay offers improved assay reliability and extends assay applicability to mixed samples. The direct, quantifiable and real time monitoring of substrate and product level makes it particularly useful for enzyme assays. For example, the activity of a kinase could be measured by monitoring the formation of a phosphorylated fluorescently labelled peptide.
詳情
基于微流體芯片的遷移率檢測技術,利用底物和產物所帶電荷的不同將兩者分離后再分別檢測,提高了分析混合樣品的可靠性和適用性。該技術可以直接、定量、實時的監測底物和產物信號的變化,特別適用于酶學分析。例如,可以通過監測磷酸化的熒光標記的底物多肽的形成來測量激酶的活性。
佰翱得使用Caliper LabChip EZ Reader II 進行激酶檢測的實例:
