表達系統
- 分類：蛋白制備
- 發布時間：2020-08-14 00:00:00
- 訪問量：0
概要:In Biortus, we utilise all major expression hosts, E.coli, yeast, insect and mammalian cells for our protein expression purposes. Since establishment, we have successfully delivered over 500 recombinant proteins, mostly enzymes and membrane proteins. The enzymes range from kinases, reductases, polymerases to epigenetic targets and others. One of the membrane proteins that we produced from scratch in four months was later crystallised by our crystallography team, adding a novel membrane protein structure to the company portfolio in a total of just eight months.
Besides conventional recombinant proteins, Biortus has protocol in place for customised protein modification such as Se-Met derivatisation, isotope labelling, biotinylation, PEG modification and more. Upon request, we can also deliver gram scale reagent grade antibodies for our clients in 6-8 weeks.
詳情
佰翱得已成熟利用所有主流表達系統來獲得目標蛋白，例如：大腸桿菌、酵母、昆蟲細胞和哺乳動物細胞表達系統。自從公司建立以來，我們已經成功表達了超過2000種重組蛋白，其中大多數是酶和膜蛋白。酶包括表觀遺傳學靶標的蛋白激酶、還原酶、聚合酶等等。并且曾經我們成功完成的一個膜蛋白項目。
除了傳統的重組蛋白之外，佰翱得還有按客戶要求定制蛋白特殊修飾的服務項目，例如硒代修飾、同位素標記、生物素化、PEG修飾等。根據要求，我們還可以在6-8周內為客戶提供克級的抗體。
